Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images were obtained with SU8010 field. Note: In this user guide, SpectraMax ® refers to several SpectraMax . 1. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax ® Pro Software. -Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). Description 1. Settings not required by a reader are indicated by ‘---’. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. com A. Five preferred detection modes. deliver increased capabilities over the M2/M2e Dual-Mode Readers at a more affordable price than the top-of-the-line M5/M5e Multi-Mode Readers, MDS Analytical Technologies can now meet an. Lower limits of detection for ATP determined using the ATPlite . SpectraMax ABS Plus SpectraTest ABS2 v1. Step 2. Find regional support. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. edu) to be added as a trained user in iLab. Softmax Pro GxP Software 6. Lift up the lid over the chamber to insert or remove a cuvette. . Bookings are free, but you will need to use iLab to book. Italic and boldface type are used for emphasis. I have been using the SpectraMax M5 since 2011. Italic and boldface type are used for emphasis. 5. no. 00 USD. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. The large analog PMT works with monochromators and is used during fluorescence measurements or when a specific luminescence wavelength is selected as in the BRET. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Some luminescence applications, such as gene reporter assays, may require a luminometer with photon counting detection for greater sensitivity, such as the SpectraMax M5, Analyst GT or Analyst HT, or LMax II. LUMINESCENCE The SpectraMax M2 and SpectraMax M2e are microplate spectrofluorometers with photomultiplier tube detection. The following document is intended to demonstrate setup of this instrument. . Open the Softmax Pro software and select the FP option. 1step assay with SpectraMax microplate readers. Currently we have over 300 Windows 7/10 compatible instrument drivers to control equipment from 147 manufacturers, meaning that we are likely to have your instrument already in our library. 305 reference level saturation. SpectraMax L Microplate Reader provides medium to high throughput. $2,264. The Spectramax can accommodate cuvettes or a variety of 6 to 96-well plate formats. . SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. 7. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. The M5 has two PMTs. Control Panel The control panel houses the instrument status LEDs and the microplate. 00 USD. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The M5 can read volumes down to 2uL with the uMax Low Volume. 2. . SoftMax Pro Software combines reader control and data analysis and provides sophisticated curve fitting and statistics for more in-depth bioassay analysis. 7°C (gradient heating design). The following document is intended to demonstrate setup of this. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. 5. These robust readers have been placed in labs from. . Plates were mixed for 2 min on an orbital shaker and then incubated at room temperature for 10 min. If you do not have an iLab account you must first make one: SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. HTRF is a proprietary time-resolved fluorescence technology that overcomes SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. The following document is intended to demonstrate setup of this instrument and provide representativeUsed together with the SpectraMax iD3 reader, the EarlyTox Cell Viability Assay Kits enable direct measurement of live and dead or apoptotic cells with a simple workflow and the increased throughput offered by a microplate format. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Qualify the fluorescence performance of the SpectraMax . Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. SpectraMax fluorescence microplate reader with SoftMax® Pro Software, is a quick, sensitive detection method for proteins. . Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Both full length human PI4KIIIB and the truncated PI4KIIIB crystal construct with the c-term were run at 200 nM. Description 1. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. . SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. All reagents were brought to room temperature before use. Wavelength optimization. For wavelengths in the UV range above 220 nm,. Molecular Devices SpectraMax M5 Multimode Microplate Reader. 0 out of 5 $ 17,999 $ 16,995. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Molecular Devices SpectraMax PLUS 384 Spectrophotometer System-New ABs1 Performance Testing August 2021. The ratioof the two fluorescence intensities 665/620 (acceptor/donor) enables the calculation of Delta F (%)As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. IMAP Assay Kits IMAP Assay Kits provide a turnkey solution to evaluate . The excitation and the emission wavelengths on. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Cytokines in medium released. A preconfiguredAcme Revival: Long Live DevicesWe would like to show you a description here but the site won’t allow us. We would like to show you a description here but the site won’t allow us. The following document is intended to demonstrate setup of this. Setup recommendations for SpectraMax® M5e. e. Bookings are. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Molecular Devices Spectramax M5 luminometer: Krishnamurthy lab-Praveen (lab WO3213, x4605) Spectramax plate reader: yes Beckman LS6000IC Scintillation counter: Bill Taylor (office WO4262B, x1966) WO3242 liquid scintillation counter Department backup -80oC freezer: Song-Tao Liu (WO4254, x7857) Facilities Facility: Department. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. Category 2 and 3 microplate readers use gradient heating as well. BRET does not work on the Spectramax M5 due to a sensitivity issue and to the fact that the BRET assay has very weak signal in general. Bookings are free, but you will need to use iLab to book. Detection of pro-inflammatory factors. . The excitation and the emission. SpectraMax DuoLuc Reporter Assay (SMP 6. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. SIRL-1 ligation reduced cytokines secretion from monocytes stimulated by NET. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. Multimode Reader Comparison Chart. 3. Three kits covering a wide range of dsDNA concentrations. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). 2. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. 1) EarlyTox Glutathione Assay (6. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Devices SpectraMax i3, SpectraMax Paradigm, or SpectraMax M5 Multi-Mode Microplate Readers. together with SpectraMax, VersaMax, VMax, and EMax microplate readers from Molecular Devices, offer a quality assurance screening solution for the detection of melamine contamination in such food products as milk, infant formula, pet food, and confectionery. SpectraMax M5 microplate reader. . The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeWe would like to show you a description here but the site won’t allow us. com A. A wireless light sensor (701-999, PS-3212) was purchased from PASCO. Set the excitation at 531 nm and emission at 595 nm. Add to Cart. Weight. 6: bioNova científica, s. Methods Preparation of reagents and standards. No filter change with dual monochromator optics. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. PAINS analysisThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. SPECTRAmax GEMINI-XS spectrofluorometer was able to detect a specific GFP signal at all cell numbers as determined by One Way Analysis of Variance (ANOVA) (Table 1). SpectraMax M3, M4, and M5/M5e readers. Samples were measured together with a corresponding reference solvent contained in a. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96. 40266 Email: drugdiscoverytech@lifetech. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. . Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Wavelength optimization. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes 6. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. It is available to all researchers. 9000-0756. Description. After 4 h, the fluorescence intensity of serum FITC-dextran was obtained at 480 nm excitation wavelength and 520 nm emission wavelength using a Spectramax M5 microplate reader (Molecular Devices). Typical LLDs were approximately 1–2 fg/well in the Analyst GT and 2–3 fg/well in the SpectraMax M5. 4. The SpectraMax ABS Plus reader utilizes Molecular . We would like to show you a description here but the site won’t allow us. If you do not have an iLab account you must first make one: Calcium Assay Kits are the platform of choice for measuring changes of intracellular calcium for drug discovery and basic research. . The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Spectranet customer portal. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. HEALTH AND SAFETY CONSIDERAIONS StakMax Handling Stacker Base Unit. Description 1. The. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 0 (IBM Corporation, Armonk, NY, USA). HTRF is a proprietary time-resolved fluorescence technology that overcomes The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Wide range of applications. ItSpectraMax® M5多功能读板机为使用者提供了除化学发光以外的其他检测方法，例如：光吸收，荧光强度，荧光偏振和时间分辨荧光。 SpectraMax® L和SpectraMax® M5酶标仪均用SoftMax® Pro软件进行操控和设置，该软件含有CellTiter-Glo检测模板和其他120多个分析模板，这些. Step 4. Wavelength Range. SoftMax Pro 5 GxP Software provides all the data collection and analysis tools of the standard edition, along withMolecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. Table of Contents. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. . KeySpan adapter for SoftMax Pro and SpectraMax readers. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. . SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices Supplier: Molecular Devices Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 00 USD. For example, microplates containing different samp les can be read using the same orProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. Microplate Reader 96 & 384 Thermo Multiskan Ascent w. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. For example, microplates containing different samples can be read using theThe interval between reads was set at 15 s or 60 s, respectively. l. . . . The optical performance is comparable to a top-of-the-line dedicated The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. 0 mL). Step 1: Open the SoftMax Pro IMAP FP protocol. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid-The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Introduction. 3: The cuvette chamber. Description 1. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Refer to “Installing Detection Cartridges” in. 1. ) Click the "OK" button on SoftMax Pro Setup dialog box . In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. The following document is intended to demonstrate setup of this instrument. Molecular Devices has collaborated with various assay partners to optimize and validate homogeneous and heterogeneous biochemical- or cell-based assay performance on the SpectraMax Microplate Reader platform. 1. The following document is intended to demonstrate setup of this instrument. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The M5 can read volumes down to 2uL with the uMax Low Volume Plate. View regional contact information and Technical Support hours: Contact Us Hi Guest, welcome to. VWR offers biophotometers for your lab's needs. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. 5. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. April 22 Asking Price: $15,990 USD Shoukat Mumtaz / Zantek Medical phone: +1 (952) 657-7977 (3). ® reader to the computer. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. 72 MB. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. Cavicide (Warehouse, Cat # 79300360) 6. With optimized reagents, option to operate the. 6. D 1 1. Published Dec 16, 2020 | Updated Dec 16, 2020. DOCUMENTATION. The chamber. 304 signal level saturation. Table of Contents. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. Agilent VWorks Automation Control soft-ware manages all processes and incubation2 Luminescence integration time For supported SpectraMax M5 microplate readers (minimum firmware requirements apply), integration times of 3, 5, 7, and 10 seconds were added for luminescence read mode. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. +1 800-635-5577. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Also refer to this Multimode Reader Comparison Chart. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. The guide also covers the software features, the specifications, and the accessories of the systems. 303 unable to cal dark current. 3: The cuvette chamber. , ~488 nm), has the optical. 1. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The plate reader can measure multiple readouts, including UV and visible absorbance. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. SpectraMax M5 Multi-Mode Microplate Reader. The absorbance and photo-luminescence was determined on a SpectraMax M5 plate reader via quartz cuvette using 1 × 10 10 particles per ml or lower and extrapolated when an optical density of 1 was. 313 reference gain check fail. D 1 1. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. 312 gain calibration failed. Sale. In this. . The assay window spanned four orders of magnitude. , ~488 nm), has the optical. Molecular Devices Spectramax M5 luminometer: Krishnamurthy lab-Praveen (lab WO3213, x4605) Spectramax plate reader: yes Beckman LS6000IC Scintillation counter: Bill Taylor (office WO4262B, x1966) WO3242 liquid scintillation counter Department backup -80oC freezer: Song-Tao Liu (WO4254, x7857) Facilities Facility: Department. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideThe SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. Choose from a three- (M3), four- (M4), orAll general chemicals and solvents were commercially available and used as received. We would like to show you a description here but the site won’t allow us. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9THE SPECTRAMAX PLUS Two ways to correct pathlength The SPECTRAmax PLUS gives you two options when making pathlength-cor-rected measurements: 1) putting the aqueous solvent into a clean 1 cm reference cuvette in the instrument’s cuvette port and having the instrument base the calcu-lations on the A 1000 and A 900Molecular Devices社の製品: マイクロプレート リーダー用ソフト SoftMax® Proに関するすべての情報をご覧ください。価格、見積もり、お近くの販売店を知るにはメーカーまたは本社に直接お問い合わせくだ. Figure 1. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Multimode Readers Comparison Chart. Highly selective for dsDNA over ssDNA or RNA. 95. For more information please visit us at: Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. Example: [Drawer]. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax Spectrophotometers for UV-Vis Absorbance Detection. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. . Optimal instrument settings are the same for all three readers. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Choose from a three- (M3), four- (M4), orThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Offer Ends In: 18 Days 19: 38: 21. Cell-based experiments In the first experiment, we prepared a dilution series of CHO-K1 cells transiently transfected withThe SpectraMax 190 reader is the tool for DNA analysis. In the M2, M5 and Plus 384, the light. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. the IMAP Platform and smoothly transition into high-throughput screening. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Cytokines in medium released. Molecular Devices Spectramax M5 Plate Reader is available from The Lab World Group. . Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. 4. acquired on a SpectraMax M5 (Molecular Devices). DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). . The Spectr. What can be done for Communication problems with new Windows 7 computer and SoftMax Pro 5. SOFTmax PRO UserÕs Manual iii Contents Figures . SpectraMax M2 and SpectraMax M2 e are controlled by an external computer running SoftMax Pro software which provides integrat ed instrument control, data display, andSpectraMax M5, Analyst HT or Analyst GT. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Customer. The following document is. For very concentrated samples, use a minimum2. 2. 5. 1) SpectraMax Glo Steady Luc Reporter Assay (6. View the Molecular Devices websiteResults by SpectraMax M5 from multiple independent donors are given. SpectraMax Spectrofluorometers. The SoftMax® Touch Software is installed in. SpectraMax M5, Analyst HT or Analyst GT. e. For more information please visit us at: Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. • Tel. 1321N1, a human astrocytoma cell line, expresses. Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The dual monochromator optics allow the widest range of applications to be utilized for bior. 1. SpectraMax® M5/M5e/FlexStation® 3 Instrument Settings Instrument Wavelength Settings EXC Wavelength 575 nm EMS Wavelength 620 nm Auto Cutoff 610 nm Optimized SpectraMax® M5/M5e Settings Readings 10-50 PMT Auto Auto Calibrate On Transcreener® HTS is a universal, high throughput biochemical assayUser guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax M5, Analyst HT or Analyst GT. Note: In this user guide, all references to SpectraMax Multi-Mode. The chamber contains. spr SpectraMax iD5 SpectraTest ABS2 v1. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4 SpectraMax® M5 Multi-Mode Microplate Reader. Fluorescence spectra of CDs were measured using a microplate reader (SpectraMax M5, Molecular Devices). e. Sale. Softmax Pro GxP Software 6. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. For more sample throughput, the system canMolecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. Absorbance (UV – VIS) Fluorescence; Glow luminescence; Time-resolved fluorescence; Fluorescent polarization; ABI Viia7 qPCR instrument (96 format) – SYBR/FAM/VIC/ROX calibrated. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. 1. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesand emission wavelengths for SpectraMax M5 fluorescence microplate readers are as follows: excitation at 490 nm and emission at 525 nm, with 515-nm emission cutoff filter (note that these are different from the wavelengths recommended for filter-based readers in the Quant-iT PicoGreen product insert). The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. 5. Wavelength Range. 2. , contact Molecular.